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superscriptiii first-strand cdna synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscriptiii first-strand cdna synthesis kit
    Superscriptiii First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscriptiii+first-strand+cdna+synthesis+kit/10__1007_slash_s11240___024___02749___x-86-12-17
    Average 90 stars, based on 1 article reviews
    superscriptiii first-strand cdna synthesis kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells
    Article Snippet: .. Isolation of messenger RNA and quantitative reverse transcription PCR Total cellular RNA from 1 × 10 6 PMA-differentiated monocyte-like THP-1 cells was extracted with TRIzol (Invitrogen Thermo Fisher Scientific, Waltham, MA) using the manufacturer instructions and reverse transcribed into complementary DNA using the SuperscriptIII First-Strand cDNA Synthesis Kit (Invitrogen Thermo Fisher Scientific) using poly dT 20 primers. .. Quantitative polymerase chain reaction (PCR) was performed using Bio-Rad IQ SYBR green supermix (Bio-Rad) in an iQ5 Real-Time PCR Detection System.

    Reverse Transcription:

    Article Title: Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells
    Article Snippet: .. Isolation of messenger RNA and quantitative reverse transcription PCR Total cellular RNA from 1 × 10 6 PMA-differentiated monocyte-like THP-1 cells was extracted with TRIzol (Invitrogen Thermo Fisher Scientific, Waltham, MA) using the manufacturer instructions and reverse transcribed into complementary DNA using the SuperscriptIII First-Strand cDNA Synthesis Kit (Invitrogen Thermo Fisher Scientific) using poly dT 20 primers. .. Quantitative polymerase chain reaction (PCR) was performed using Bio-Rad IQ SYBR green supermix (Bio-Rad) in an iQ5 Real-Time PCR Detection System.

    Article Title: Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells
    Article Snippet: .. Total cellular RNA from 1 × 10 6 PMA-differentiated monocyte-like THP-1 cells was extracted with TRIzol (Invitrogen Thermo Fisher Scientific, Waltham, MA) using the manufacturer instructions and reverse transcribed into complementary DNA using the SuperscriptIII First-Strand cDNA Synthesis Kit (Invitrogen Thermo Fisher Scientific) using poly dT 20 primers. .. Quantitative polymerase chain reaction (PCR) was performed using Bio-Rad IQ SYBR green supermix (Bio-Rad) in an iQ5 Real-Time PCR Detection System.

    Article Title: Chloral hydrate decreases gap junction communication in rat liver epithelial cells.
    Article Snippet: Gap junction communication (GJC) is involved in controlling cell proliferation and differentiation.. Alterations in GJC are associated with carcinogenesis, but the mechanisms involved are unknown.. Chloral hydrate (CH), a by-product of chlorine disinfection of water, is carcinogenic in mice, and we demonstrated that CH reduced GJC in a rat liver epithelial cell line (Clone 9).

    Polymerase Chain Reaction:

    Article Title: Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells
    Article Snippet: .. Isolation of messenger RNA and quantitative reverse transcription PCR Total cellular RNA from 1 × 10 6 PMA-differentiated monocyte-like THP-1 cells was extracted with TRIzol (Invitrogen Thermo Fisher Scientific, Waltham, MA) using the manufacturer instructions and reverse transcribed into complementary DNA using the SuperscriptIII First-Strand cDNA Synthesis Kit (Invitrogen Thermo Fisher Scientific) using poly dT 20 primers. .. Quantitative polymerase chain reaction (PCR) was performed using Bio-Rad IQ SYBR green supermix (Bio-Rad) in an iQ5 Real-Time PCR Detection System.

    cDNA Synthesis:

    Article Title: Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells
    Article Snippet: .. Isolation of messenger RNA and quantitative reverse transcription PCR Total cellular RNA from 1 × 10 6 PMA-differentiated monocyte-like THP-1 cells was extracted with TRIzol (Invitrogen Thermo Fisher Scientific, Waltham, MA) using the manufacturer instructions and reverse transcribed into complementary DNA using the SuperscriptIII First-Strand cDNA Synthesis Kit (Invitrogen Thermo Fisher Scientific) using poly dT 20 primers. .. Quantitative polymerase chain reaction (PCR) was performed using Bio-Rad IQ SYBR green supermix (Bio-Rad) in an iQ5 Real-Time PCR Detection System.

    Article Title: The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress.
    Article Snippet: .. An aliquot of 2 mg of mRNA from each sample was used to synthesize the first-strand cDNA using the SuperScriptIII first-strand cDNA synthesis kit (Life Technologies). ..

    Article Title: Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells
    Article Snippet: .. Total cellular RNA from 1 × 10 6 PMA-differentiated monocyte-like THP-1 cells was extracted with TRIzol (Invitrogen Thermo Fisher Scientific, Waltham, MA) using the manufacturer instructions and reverse transcribed into complementary DNA using the SuperscriptIII First-Strand cDNA Synthesis Kit (Invitrogen Thermo Fisher Scientific) using poly dT 20 primers. .. Quantitative polymerase chain reaction (PCR) was performed using Bio-Rad IQ SYBR green supermix (Bio-Rad) in an iQ5 Real-Time PCR Detection System.

    Article Title: Indexing heat stress induced changes in transgenic tobacco by overexpressing membrane-tethered transcription factor from Sorghum bicolor bZIP17
    Article Snippet: Rapidly changing global climate conditions result in different abiotic stresses, especially heat stress which is a major constraint for future food security.. Heat stress hampers physiological and biochemical processes by negatively affecting crop growth, development, and ultimately yield (Saeed et al. 2023).. Plants have evolved a variety of tolerance mechanism in response to heat stress conditions and undergo various physiological, biochemical, and molecular modifications for adaptation (Janni et al. 2020; Saeed et al. 2023).

    Article Title: Chloral hydrate decreases gap junction communication in rat liver epithelial cells.
    Article Snippet: Gap junction communication (GJC) is involved in controlling cell proliferation and differentiation.. Alterations in GJC are associated with carcinogenesis, but the mechanisms involved are unknown.. Chloral hydrate (CH), a by-product of chlorine disinfection of water, is carcinogenic in mice, and we demonstrated that CH reduced GJC in a rat liver epithelial cell line (Clone 9).

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer’s instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20 ; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm004330 11_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm0043 8670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer's instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20×; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm00433011_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm00438670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Synthesized:

    Article Title: Indexing heat stress induced changes in transgenic tobacco by overexpressing membrane-tethered transcription factor from Sorghum bicolor bZIP17
    Article Snippet: Rapidly changing global climate conditions result in different abiotic stresses, especially heat stress which is a major constraint for future food security.. Heat stress hampers physiological and biochemical processes by negatively affecting crop growth, development, and ultimately yield (Saeed et al. 2023).. Plants have evolved a variety of tolerance mechanism in response to heat stress conditions and undergo various physiological, biochemical, and molecular modifications for adaptation (Janni et al. 2020; Saeed et al. 2023).

    Purification:

    Article Title: Chloral hydrate decreases gap junction communication in rat liver epithelial cells.
    Article Snippet: Gap junction communication (GJC) is involved in controlling cell proliferation and differentiation.. Alterations in GJC are associated with carcinogenesis, but the mechanisms involved are unknown.. Chloral hydrate (CH), a by-product of chlorine disinfection of water, is carcinogenic in mice, and we demonstrated that CH reduced GJC in a rat liver epithelial cell line (Clone 9).

    RNA Extraction:

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer’s instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20 ; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm004330 11_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm0043 8670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer's instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20×; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm00433011_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm00438670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Random Hexamer:

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer’s instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20 ; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm004330 11_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm0043 8670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer's instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20×; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm00433011_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm00438670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Quantitative RT-PCR:

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer’s instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20 ; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm004330 11_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm0043 8670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    Article Title: EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche
    Article Snippet: .. The RMS was dissected from two male mice, minced, and combined followed by RNA extraction using the RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. cDNA was prepared with the SuperScriptIII First-Strand cDNA synthesis kit (Thermo Fisher Scientific) using random hexamer primers according to the manufacturer's instructions. qRT-PCR was performed on a Bio-Rad C1000 thermal cycler with CFX96 Real-Time system using the following mouse specific TaqMan assays (20×; Thermo Fisher Scientific): Gapdh (Mm99999915_g1), EPHA4 (Mm00433056_m1), EFNA1 (Mm01212795_m1), EFNA2 (Mm00433011_m1), EFNA3 (Mm01212723_g1), EFNA4 (Mm00433013_m1), EFNA5 (Mm01237700_m1), EFNB1 (Mm00438666_m1), EFNB2 (Mm00438670_m1), and EFNB3 (Mm00433016_m1). .. Data were analyzed with the Bio-Rad CFX Manager software.

    other:

    Article Title: Gabra5-gene haplotype block associated with behavioral properties of the full agonist benzodiazepine chlordiazepoxide.
    Article Snippet: The gabra5 gene is associated with pharmacological properties (myorelaxant, amnesic, anxiolytic) of benzodiazepines.. It is tightly located (0.5 cM) close to the pink-eyed dilution (p) locus which encodes for fur color on mouse chromosome 7.. We tested the putative role of the gabra5 gene in pharmacological properties of the full non specific agonist chlordiazepoxide (CDP), using behavioral and molecular approaches in mutated p/p mice and wild type F2 from crosses between two multiple markers inbred strain ABP/Le and C57BL/6By strain.



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